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In vitro characterization of the fibroin gene promoter by the use of single-base substitution mutants.
A highly efficient method for segment-directed mutagenesis has been developed. The method relies on the deamination by sodium nitrite of the bases in the separated strands of a small DNA restriction fragment. The mutagen-treated strands produce transition mutations by the following sequence: (i) hyb...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1982
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC347318/ https://ncbi.nlm.nih.gov/pubmed/6961405 |
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