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In vitro characterization of the fibroin gene promoter by the use of single-base substitution mutants.
A highly efficient method for segment-directed mutagenesis has been developed. The method relies on the deamination by sodium nitrite of the bases in the separated strands of a small DNA restriction fragment. The mutagen-treated strands produce transition mutations by the following sequence: (i) hyb...
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| Veröffentlicht in: | Proc Natl Acad Sci U S A |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
National Academy of Sciences
1982
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC347318/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6961405/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.23.7258 |
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