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Transformation and replication in mouse cells of a bovine papillomavirus--pML2 plasmid vector that can be rescued in bacteria.

The unique ability of bovine papillomavirus (BPV-1) DNA to replicate as a stable, multicopy plasmid in transformed mouse cells has led to its utilization as a eukaryotic cloning vector. One limitation of the system has been the marked reduction in transformation efficiency when BPV-1 DNA or the subg...

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Bibliographic Details
Published in:Proc Natl Acad Sci U S A
Main Authors: Sarver, N, Byrne, J C, Howley, P M
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 1982
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Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC347295/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6296820/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.23.7147
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