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Measuring Intermolecular Rupture Forces with a Combined TIRF-Optical Trap Microscope and DNA Curtains

We report a new approach to probing DNA-protein interactions by combining optical tweezers with a high-throughput DNA curtains technique. Here we determine the forces required to remove the individual lipid-anchored DNA molecules from the bilayer. We demonstrate that DNA anchored to the bilayer thro...

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Detalhes bibliográficos
Main Authors: Lee, Ja Yil, Wang, Feng, Fazio, Teresa, Wind, Shalom, Greene, Eric
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3467351/
https://ncbi.nlm.nih.gov/pubmed/22967893
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2012.08.127
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