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Measuring Intermolecular Rupture Forces with a Combined TIRF-Optical Trap Microscope and DNA Curtains
We report a new approach to probing DNA-protein interactions by combining optical tweezers with a high-throughput DNA curtains technique. Here we determine the forces required to remove the individual lipid-anchored DNA molecules from the bilayer. We demonstrate that DNA anchored to the bilayer thro...
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| Glavni autori: | , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2012
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3467351/ https://ncbi.nlm.nih.gov/pubmed/22967893 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2012.08.127 |
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