Functional origin of replication of pT181 plasmid DNA is contained within a 168-base-pair segment.
We have used a recently developed in vitro replication system from Staphylococcus aureus to determine the origin and direction of replication of pT181 plasmid DNA. The origin was located to within 168 base pairs by two methods: (i) sequential labeling of restriction endonuclease fragments after sync...
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| 發表在: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
National Academy of Sciences
1982
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC346718/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6956881/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.15.4580 |
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