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‘Shotgun DNA synthesis’ for the high-throughput construction of large DNA molecules
We developed a highly scalable ‘shotgun’ DNA synthesis technology by utilizing microchip oligonucleotides, shotgun assembly and next-generation sequencing technology. A pool of microchip oligonucleotides targeting a penicillin biosynthetic gene cluster were assembled into numerous random fragments,...
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| Main Authors: | , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3467036/ https://ncbi.nlm.nih.gov/pubmed/22705793 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks546 |
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