‘Shotgun DNA synthesis’ for the high-throughput construction of large DNA molecules

We developed a highly scalable ‘shotgun’ DNA synthesis technology by utilizing microchip oligonucleotides, shotgun assembly and next-generation sequencing technology. A pool of microchip oligonucleotides targeting a penicillin biosynthetic gene cluster were assembled into numerous random fragments,...

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Hlavní autoři: Kim, Hwangbeom, Han, Hyojun, Ahn, Jinwoo, Lee, Joongoo, Cho, Namjin, Jang, Hoon, Kim, Hyoki, Kwon, Sunghoon, Bang, Duhee
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2012
Témata:
Methods Online
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3467036/
https://ncbi.nlm.nih.gov/pubmed/22705793
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks546
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