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Pilot production of u-PA with porous microcarrier cell culture
A recombinant DNA CHO cell line secretingurokinase-type plasminogen activator (u-PA) wascultivated with Cytopore cellulose porousmicrocarriers in a 30l Biostat UC stirred tankreactor. After 26 days of culture, using a spinfilter toretain cells in bioreactor, the cell density couldreach 1.33 × 10(7)...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Kluwer Academic Publishers
2000
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3466703/ https://ncbi.nlm.nih.gov/pubmed/19002806 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1008127310890 |
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