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Control of transmembrane ion fluxes to select halorhodopsin-deficient and other energy-transduction mutants of Halobacterium halobium.

We describe a selection method for mutants altered in the generation and regulation of transmembrane ion flux in Halobacterium halobium. The method is based on experimental control of ion fluxes by a combination of light, ionophore, and external pH to generate an imbalance in the cells' proton...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Spudich, E N, Spudich, J L
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1982
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC346660/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6289299/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.14.4308
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