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A pentacyclic reaction intermediate of riboflavin synthase
The S41A mutant of riboflavin synthase from Escherichia coli catalyzes the formation of riboflavin from 6,7-dimethyl-8-ribityllumazine at a very low rate. Quenching of presteady-state reaction mixtures with trifluoroacetic acid afforded a compound with an absorption maximum at 412 nm (pH 1.0) that c...
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Main Authors: | , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
The National Academy of Sciences
2001
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC34650/ https://ncbi.nlm.nih.gov/pubmed/11404482 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.131610698 |
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