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DNA-mediated gene transfer: recombination between cotransferred DNA sequences and recovery of recombinants in a plasmid.
Ltk- aprt- mouse L cells were transformed to the tk+ phenotype with 10 ng of the herpes simplex virus-1 thymidine kinase (tk) gene and 20 micrograms of pBR322 or simian virus 40 (SV40) DNA. DNAs from five cloned cell lines show restriction endonuclease fragments that hybridize to both tk and pBR322...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1982
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC346283/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6283542/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.9.2748 |
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