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Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag

Due to the high costs associated with purification of recombinant proteins the protocols need to be rationalized. For high-throughput efforts there is a demand for general methods that do not require target protein specific optimization(1) . To achieve this, purification tags that genetically can be...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Nilvebrant, Johan, Alm, Tove, Hober, Sophia
Format: Artigo
Sprache:Inglês
Veröffentlicht: MyJove Corporation 2012
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3462565/
https://ncbi.nlm.nih.gov/pubmed/22297419
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/3370
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