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Cloning of adeno-associated virus into pBR322: rescue of intact virus from the recombinant plasmid in human cells.
We have cloned intact duplex adeno-associated virus (AAV) DNA into the bacterial plasmid pBR322. The AAV genome could be rescued from the recombinant plasmid by transfection of the plasmid DNA into human cells with adenovirus 5 as helper. The efficiency of rescue from the plasmid was sufficiently hi...
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| Publicado no: | Proc Natl Acad Sci U S A |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1982
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC346126/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6281795/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.6.2077 |
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