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Amplification of expression of hepatitis B surface antigen in 3T3 cells cotransfected with a dominant-acting gene and cloned viral DNA.

3T3 cells containing hepatitis B virus DNA sequences can be efficiently selected by exposure to methotrexate after cotransfection with cloned viral DNA and DNA coding for a methotrexate-resistant dihydrofolate reductase. More than 75% of methotrexate-resistant cells isolated after cotransfection wit...

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Hlavní autoři: Christman, J K, Gerber, M, Price, P M, Flordellis, C, Edelman, J, Acs, G
Médium: Artigo
Jazyk:Inglês
Vydáno: 1982
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC346071/
https://ncbi.nlm.nih.gov/pubmed/6952231
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