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Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

A protocol has been developed to overcome the difficulties of isolating and characterizing rare T cells specific for pathogens, such as human papillomavirus (HPV), that cause localized infections. The steps involved are identifying region(s) of HPV proteins that contain T-cell epitope(s) from a subj...

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Main Authors: Wang, Xuelian, Greenfield, William W., Coleman, Hannah N., James, Lindsey E., Nakagawa, Mayumi
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2012
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3460593/
https://ncbi.nlm.nih.gov/pubmed/22434036
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/3657
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