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Mapping of the vaccinia virus thymidine kinase gene by marker rescue and by cell-free translation of selected mRNA

A selective plaque assay that uses thymidine kinase (TK)-deficient human 143 cells was developed to titer mixtures of TK(+) and TK(-) vaccinia virus. With this assay it could be shown that methotrexate-resistant TK(+) virus was formed in cells coinfected with TK(-) virus and wild-type virus DNA. By...

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Bibliografische gegevens
Hoofdauteurs: Weir, Jerry P., Bajszár, Gyorgy, Moss, Bernard
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1982
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC345931/
https://ncbi.nlm.nih.gov/pubmed/6280173
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