Effect of methylation on expression of microinjected genes.
The cloned genes for the simian virus 40 large tumor antigen and for herpes simplex virus (HSV) thymidine kinase (TK) were methylated with EcoRI methylase. The genes were microinjected into the nuclei of TK-deficient (tk-) cells, and expression of the genes was determined by immunofluorescence stain...
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| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1982
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC345909/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6280167/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.4.1106 |
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