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Dynamics of transcription driven by the tetA promoter, one event at a time, in live Escherichia coli cells

In Escherichia coli, tetracycline prevents translation. When subject to tetracycline, E. coli express TetA to pump it out by a mechanism that is sensitive, while fairly independent of cellular metabolism. We constructed a target gene, P(tetA)-mRFP1-96BS, with a 96 MS2-GFP binding site array in a sin...

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Detalhes bibliográficos
Main Authors: Muthukrishnan, Anantha-Barathi, Kandhavelu, Meenakshisundaram, Lloyd-Price, Jason, Kudasov, Fedor, Chowdhury, Sharif, Yli-Harja, Olli, Ribeiro, Andre S.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3458540/
https://ncbi.nlm.nih.gov/pubmed/22730294
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks583
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