Antibody-free, targeted mass-spectrometric approach for quantification of proteins at low picogram per milliliter levels in human plasma/serum

Sensitive detection of low-abundance proteins in complex biological samples has typically been achieved by immunoassays that use antibodies specific to target proteins; however, de novo development of antibodies is associated with high costs, long development lead times, and high failure rates. To a...

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Bibliografiske detaljer
Main Authors: Shi, Tujin, Fillmore, Thomas L., Sun, Xuefei, Zhao, Rui, Schepmoes, Athena A., Hossain, Mahmud, Xie, Fang, Wu, Si, Kim, Jong-Seo, Jones, Nathan, Moore, Ronald J., Paša-Tolić, Ljiljana, Kagan, Jacob, Rodland, Karin D., Liu, Tao, Tang, Keqi, Camp, David G., Smith, Richard D., Qian, Wei-Jun
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2012
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3458402/
https://ncbi.nlm.nih.gov/pubmed/22949669
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1204366109
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