Counterselection System for Geobacillus kaustophilus HTA426 through Disruption of pyrF and pyrR

Counterselection systems facilitate marker-free genetic modifications in microbes by enabling positive selections for both the introduction of a marker gene into the microbe and elimination of the marker from the microbe. Here we report a counterselection system for Geobacillus kaustophilus HTA426,...

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Bibliographic Details
Main Authors: Suzuki, Hirokazu, Murakami, Ayano, Yoshida, Ken-ichi
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2012
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Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3457123/
https://ncbi.nlm.nih.gov/pubmed/22885745
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01669-12
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