Optimal conditions for cell-free synthesis of citrus exocortis viroid and the question of specificity of RNA polymerase activity
Cell-free synthesis of citrus exocortis viroid (CEV) in nuclei-rich preparations from infected Gynura aurantiaca was optimum at 18-24°C. Incubation of reaction mixtures at higher temperatures (30-36°C) resulted in an increase of CEV linear molecules and the recovery of incomplete or nicked newly syn...
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| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1984
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC345603/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16593489/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.14.4429 |
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