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Optimal conditions for cell-free synthesis of citrus exocortis viroid and the question of specificity of RNA polymerase activity
Cell-free synthesis of citrus exocortis viroid (CEV) in nuclei-rich preparations from infected Gynura aurantiaca was optimum at 18-24°C. Incubation of reaction mixtures at higher temperatures (30-36°C) resulted in an increase of CEV linear molecules and the recovery of incomplete or nicked newly syn...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1984
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC345603/ https://ncbi.nlm.nih.gov/pubmed/16593489 |
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