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Comparison of the Media for Isolation and Storage of Round Spermatid Nuclei Before Intracytoplasmic Injection

Purpose:This study investigated whether K(+)-rich medium is better than pure NaCl solution or Na(+)-rich cell culture medium for handling round spermatid nuclei prior to injection into oocytes (ROSNI). Methods:Round spermatids of the mouse were isolated and stored in isotonic NaCl, a cell culture me...

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Bibliografski detalji
Glavni autori: Suzuki, Kazuo, Yanagida, Kaoru, Yanagimachi, R.
Format: Artigo
Jezik:Inglês
Izdano: Kluwer Academic Publishers-Plenum Publishers 1998
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3454979/
https://ncbi.nlm.nih.gov/pubmed/9547692
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1023013022295
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