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Comparison of the Media for Isolation and Storage of Round Spermatid Nuclei Before Intracytoplasmic Injection
Purpose:This study investigated whether K(+)-rich medium is better than pure NaCl solution or Na(+)-rich cell culture medium for handling round spermatid nuclei prior to injection into oocytes (ROSNI). Methods:Round spermatids of the mouse were isolated and stored in isotonic NaCl, a cell culture me...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Kluwer Academic Publishers-Plenum Publishers
1998
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3454979/ https://ncbi.nlm.nih.gov/pubmed/9547692 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1023013022295 |
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