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Construction of mutants of Moloney murine leukemia virus by suppressor-linker insertional mutagenesis: positions of viable insertion mutations.

A highly efficient method for the generation of insertion mutations is described. The procedure involves the use of a 220-base-pair (bp) EcoRI fragment bearing the SuIII+ suppressor tRNA gene as an insertional mutagen. The plasmid DNA to be mutagenized is linearized by a variety of means, and the su...

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Detalles Bibliográficos
Autores principales: Lobel, L I, Goff, S P
Formato: Artigo
Lenguaje:Inglês
Publicado: 1984
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC345386/
https://ncbi.nlm.nih.gov/pubmed/6330745
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