Construction of mutants of Moloney murine leukemia virus by suppressor-linker insertional mutagenesis: positions of viable insertion mutations.

A highly efficient method for the generation of insertion mutations is described. The procedure involves the use of a 220-base-pair (bp) EcoRI fragment bearing the SuIII+ suppressor tRNA gene as an insertional mutagen. The plasmid DNA to be mutagenized is linearized by a variety of means, and the su...

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Veröffentlicht in:	Proc Natl Acad Sci U S A
Hauptverfasser:	Lobel, L I, Goff, S P
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	National Academy of Sciences 1984
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC345386/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6330745/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.13.4149
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