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Differential antibody screening of cloned Plasmodium falciparum sequences expressed in Escherichia coli: procedure for isolation of defined antigens and analysis of human antisera.

We describe a procedure that uses polyspecific human sera for screening Escherichia coli colonies expressing cloned Plasmodium falciparum cDNA sequences in order to detect colonies that react differentially with different sera. This procedure can be used for two distinct purposes. First, it enables...

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Bibliografische Detailangaben
Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: Stahl, H D, Coppel, R L, Brown, G V, Saint, R, Lingelbach, K, Cowman, A F, Anders, R F, Kemp, D J
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 1984
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC345080/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6371814/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.8.2456
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