Specific amino acid substitutions in bacterioopsin: Replacement of a restriction fragment in the structural gene by synthetic DNA fragments containing altered codons

To study the mechanism of light-dependent proton translocation by bacteriorhodopsin, we have introduced single-codon changes in the gene so as to produce the following specific amino acid substitutions in the protein: Tyr-185 to Phe, Pro-186 to Leu, Trp-189 to Phe, Ser-193 to Ala, and Glu-194 to Gln...

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Veröffentlicht in:	Proc Natl Acad Sci U S A
Hauptverfasser:	Lo, Kin-Ming, Jones, Simon S., Hackett, Neil R., Khorana, H. Gobind
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	National Academy of Sciences 1984
Schlagworte:	Biological Sciences: Biochemistry
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC345043/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16593452/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.8.2285
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