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Structural and biochemical properties of lichenase from Clostridium thermocellum
The recombinant enzyme lichenase of size 30 kDa was over-expressed using E. coli cells and purified by immobilized metal ion affinity chromatography (IMAC) and size exclusion chromatography. The enzyme displayed high activity towards lichenan and β-glucan. The enzyme showed no activity towards carbo...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Springer-Verlag
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3450042/ https://ncbi.nlm.nih.gov/pubmed/23100753 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12088-009-0003-3 |
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