Structural and biochemical properties of lichenase from Clostridium thermocellum

The recombinant enzyme lichenase of size 30 kDa was over-expressed using E. coli cells and purified by immobilized metal ion affinity chromatography (IMAC) and size exclusion chromatography. The enzyme displayed high activity towards lichenan and β-glucan. The enzyme showed no activity towards carbo...

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Bibliographische Detailangaben
Hauptverfasser: Ahmed, Shadab, Bharali, Sangeeta, Purama, Ravi Kiran, Majumder, Avishek, Fontes, Carlos M. G. A., Goyal, Arun
Format: Artigo
Sprache:Inglês
Veröffentlicht: Springer-Verlag 2009
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Original Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3450042/
https://ncbi.nlm.nih.gov/pubmed/23100753
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12088-009-0003-3
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