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A quantitative dot-immunobinding assay for proteins using nitrocellulose membrane filters.

An immunoassay method is described for the quantitative determination of synapsin I (protein I) and of a 36,000-dalton membrane protein from rat brain synaptic vesicles. The samples are spotted on nitrocellulose membrane filters, incubated sequentially with specific antibodies and 125I-labeled prote...

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Bibliografske podrobnosti
izdano v:Proc Natl Acad Sci U S A
Principais autores: Jahn, R, Schiebler, W, Greengard, P
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 1984
Teme:
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC344982/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6424121/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.6.1684
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