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In vitro immunization of human peripheral blood lymphocytes: establishment of B cell lines secreting IgM specific for cholera toxin B subunit from lymphocytes stimulated with IL-2 and IL-4

In vitro immunization (IVI) techniques have a great potential in the production of human monoclonal antibodies (MAbs) against various antigens. An IVI method of human peripheral blood lymphocytes (PBL) has been developed with a human lung adenocarcinoma cell line in our laboratory. Although several...

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Autori principali: Ichikawa, Akira, Katakura, Yoshinori, Teruya, Kiichiro, Hashizume, Shuichi, Shirahata, Sanetaka
Natura: Artigo
Lingua:Inglês
Pubblicazione: Kluwer Academic Publishers 1999
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3449779/
https://ncbi.nlm.nih.gov/pubmed/19003133
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1008063903946
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