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Preparation of Secretory Vesicle-Free Plasma Membranes by Isopycnic Sucrose Gradient Fractionation of Neutrophils Purified by the Gelatin Method
Isolated human neutrophils serve as a model for the in vitro study of host defensive processes as well as the cell biology and biochemistry of primary human cells. We demonstrate that the requirements of the gelatinbased procedure for neutrophil isolation from whole blood induces the complete loss o...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Kluwer Academic Publishers
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3449717/ https://ncbi.nlm.nih.gov/pubmed/19003266 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-005-0300-6 |
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