Optimizing the stability of single-chain proteins by linker length and composition mutagenesis
Linker length and composition were varied in libraries of single-chain Arc repressor, resulting in proteins with effective concentrations ranging over six orders of magnitude (10 μM–10 M). Linkers of 11 residues or more were required for biological activity. Equilibrium stability varied substantiall...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1998
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC34497/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9600894/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.95.11.5929 |
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