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Alteration of mammalian cell metabolism by dynamic nutrient feeding
The metabolism of hybridoma cells was controlled to reduce metabolic formation in fed-batch cultures by dynamically feeding a salt-free nutrient concentrate. For this purpose, on-line oxygen uptake rate (OUR) measurement was used to estimate the metabolic demand of hybridoma cells and to determine t...
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| Главные авторы: | , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Kluwer Academic Publishers
1997
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3449580/ https://ncbi.nlm.nih.gov/pubmed/22358650 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1007945826228 |
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