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Production of a self-activating CBM-factor X fusion protein in a stable transformed Sf9 insect cell line using high cell density perfusion culture

Factor Xa is a serine protease, whose high selectivity can be used to cleave protein tags from recombinant proteins. A fusion protein comprised of a self-activating form of factor X linked to a cellulose-binding module, saCBMFX, was produced in a stable transformed Sf9 insect cell line. The activity...

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Detalhes bibliográficos
Main Authors: Gorenflo, Volker M., Pfeifer, Tom A., Lesnicki, Gary, Kwan, Emily M., Grigliatti, Thomas A., Kilburn, Douglas G., Piret, James M.
Formato: Artigo
Idioma:Inglês
Publicado em: Kluwer Academic Publishers 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3449484/
https://ncbi.nlm.nih.gov/pubmed/19003232
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-005-0703-4
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