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A method to maintain mammalian cells for days alive at 4 °C

This paper describes a method for the temporary storage of cultured cells. Cells from recently completed cell monolayers were trypsinized and then centrifuged. After centrifugation, the supernatant and pellet were kept at 4 °C for one week. After storage, the supernatant was discarded, the cells wer...

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Détails bibliographiques
Auteurs principaux: Mocé-Llivina, Laura, Jofre, Juan
Format: Artigo
Langue:Inglês
Publié: Kluwer Academic Publishers 2005
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3449471/
https://ncbi.nlm.nih.gov/pubmed/19003259
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-005-2106-y
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