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An engineered eukaryotic protein glycosylation pathway in Escherichia coli
We performed bottom-up engineering of a synthetic pathway in E. coli for the production of eukaryotic trimannosyl chitobiose glycans and the transfer of these glycans to specific asparagine residues in target proteins. Glycan biosynthesis was enabled by four eukaryotic glycosyltransferases, includin...
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| Main Authors: | , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3449280/ https://ncbi.nlm.nih.gov/pubmed/22446837 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.921 |
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