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Kinetic Analysis of Guanidine Hydrochloride Inactivation of β-Galactosidase in the Presence of Galactose

Inactivation of purified β-Galactosidase was done with GdnHCl in the absence and presence of varying [galactose] at 50°C and at pH 4.5. Lineweaver-Burk plots of initial velocity data, in the presence and absence of guanidine hydrochloride (GdnHCl) and galactose, were used to determine the relevant K...

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Detaylı Bibliyografya
Asıl Yazarlar: Nwamba, Charles O., Chilaka, Ferdinand C.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Hindawi Publishing Corporation 2012
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC3449116/
https://ncbi.nlm.nih.gov/pubmed/23008759
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2012/173831
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