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Covalent attachment of heme to the protein moiety in an insect E75 nitric oxide sensor
We have recombinantly expressed and purified the ligand binding domains (LBDs) of four insect nuclear receptors of the E75 family. The Drosophila melanogaster and Bombyx mori nuclear receptors were purified as ferric hemoproteins with Soret maxima at 424 nm, whereas their ferrous form had a Soret ma...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3448046/ https://ncbi.nlm.nih.gov/pubmed/22946928 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi300848x |
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