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Application of non-negative matrix factorization to multispectral FLIM data analysis

Existing methods of interpreting fluorescence lifetime imaging microscopy (FLIM) images are based on comparing the intensity and lifetime values at each pixel with those of known fluorophores. This method becomes unwieldy and subjective in many practical applications where there are several fluoresc...

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Detalhes bibliográficos
Main Authors: Pande, Paritosh, Applegate, Brian E., Jo, Javier A.
Formato: Artigo
Idioma:Inglês
Publicado em: Optical Society of America 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3447565/
https://ncbi.nlm.nih.gov/pubmed/23024917
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.3.002244
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