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Construction and characterization of extrachromosomal probes for mutagenesis by carcinogens: site-specific incorporation of O6-methylguanine into viral and plasmid genomes.

Organic synthesis and recombinant DNA technology were used to situate a putatively premutagenic DNA lesion, O6-methylguanine (O6MeGua), at a specific location in the genomes of two bacterial viruses, M13mp8 and phi X174, and of the bacterial plasmid pBR322. In each genome the first guanine residue i...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Green, C L, Loechler, E L, Fowler, K W, Essigmann, J M
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1984
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC344600/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6320161/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.1.13
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