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Quantitative Fluorescent Labeling of Aldehyde-Tagged Proteins for Single-Molecule Imaging
A major hurdle for molecular mechanistic studies of many proteins is the lack of a general method for fluorescent labeling with high efficiency, specificity, and speed. By incorporating an aldehyde motif genetically into a protein and improving the labeling kinetics substantially under mild conditio...
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| 主要な著者: | , , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2012
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3445270/ https://ncbi.nlm.nih.gov/pubmed/22466795 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1954 |
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