Fully Automated, Internally Controlled Quantification of Hepatitis B Virus DNA by Real-Time PCR by Use of the MagNA Pure LC and LightCycler Instruments

We report on the development of a fully automated real-time PCR assay for the quantitative detection of hepatitis B virus (HBV) DNA in plasma with EDTA (EDTA plasma). The MagNA Pure LC instrument was used for automated DNA purification and automated preparation of PCR mixtures. Real-time PCR was per...

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Detalhes bibliográficos
Main Authors: Leb, Victoria, Stöcher, Markus, Valentine-Thon, Elizabeth, Hölzl, Gabriele, Kessler, Harald, Stekel, Herbert, Berg, Jörg
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Virology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC344446/
https://ncbi.nlm.nih.gov/pubmed/14766820
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.2.585-590.2004
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