Smallpox and pan-Orthopox Virus Detection by Real-Time 3′-Minor Groove Binder TaqMan Assays on the Roche LightCycler and the Cepheid Smart Cycler Platforms

We designed, optimized, and extensively tested several sensitive and specific real-time PCR assays for rapid detection of both smallpox and pan-orthopox virus DNAs. The assays are based on TaqMan 3′-minor groove binder chemistry and were performed on both the rapid-cycling Roche LightCycler and the...

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Main Authors: Kulesh, David A., Baker, Robert O., Loveless, Bonnie M., Norwood, David, Zwiers, Susan H., Mucker, Eric, Hartmann, Chris, Herrera, Rafael, Miller, David, Christensen, Deanna, Wasieloski, Leonard P., Huggins, John, Jahrling, Peter B.
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 2004
主題:
Virology
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC344443/
https://ncbi.nlm.nih.gov/pubmed/14766823
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.2.601-609.2004
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