Screening a cDNA Library for Protein–Protein Interactions Directly in Planta([W])

Screening cDNA libraries for genes encoding proteins that interact with a bait protein is usually performed in yeast. However, subcellular compartmentation and protein modification may differ in yeast and plant cells, resulting in misidentification of protein partners. We used bimolecular fluorescen...

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Auteurs principaux: Lee, Lan-Ying, Wu, Fu-Hui, Hsu, Chen-Tran, Shen, Shu-Chen, Yeh, Hsuan-Yu, Liao, De-Chih, Fang, Mei-Jane, Liu, Nien-Tze, Yen, Yu-Chen, Dokládal, Ladislav, Sýkorová, Eva, Gelvin, Stanton B., Lin, Choun-Sea
Format: Artigo
Langue:Inglês
Publié: American Society of Plant Biologists 2012
Sujets:
Large-Scale Biology Articles
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3442567/
https://ncbi.nlm.nih.gov/pubmed/22623495
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1105/tpc.112.097998
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