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Highly Efficient Isolation of Populus Mesophyll Protoplasts and Its Application in Transient Expression Assays
BACKGROUND: Populus is a model woody plant and a promising feedstock for lignocellulosic biofuel production. However, its lengthy life cycle impedes rapid characterization of gene function. METHODOLOGY/PRINCIPAL FINDINGS: We optimized a Populus leaf mesophyll protoplast isolation protocol and establ...
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Main Authors: | , , , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Public Library of Science
2012
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3441479/ https://ncbi.nlm.nih.gov/pubmed/23028673 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0044908 |
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