Highly Efficient Isolation of Populus Mesophyll Protoplasts and Its Application in Transient Expression Assays

BACKGROUND: Populus is a model woody plant and a promising feedstock for lignocellulosic biofuel production. However, its lengthy life cycle impedes rapid characterization of gene function. METHODOLOGY/PRINCIPAL FINDINGS: We optimized a Populus leaf mesophyll protoplast isolation protocol and establ...

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Main Authors: Guo, Jianjun, Morrell-Falvey, Jennifer L., Labbé, Jessy L., Muchero, Wellington, Kalluri, Udaya C., Tuskan, Gerald A., Chen, Jin-Gui
格式: Artigo
語言:Inglês
出版: Public Library of Science 2012
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Research Article
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3441479/
https://ncbi.nlm.nih.gov/pubmed/23028673
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0044908
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