Efficient and robust RNA-seq process for cultured bacteria and complex community transcriptomes

We have developed a process for transcriptome analysis of bacterial communities that accommodates both intact and fragmented starting RNA and combines efficient rRNA removal with strand-specific RNA-seq. We applied this approach to an RNA mixture derived from three diverse cultured bacterial species...

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Detalhes bibliográficos
Main Authors: Giannoukos, Georgia, Ciulla, Dawn M, Huang, Katherine, Haas, Brian J, Izard, Jacques, Levin, Joshua Z, Livny, Jonathan, Earl, Ashlee M, Gevers, Dirk, Ward, Doyle V, Nusbaum, Chad, Birren, Bruce W, Gnirke, Andreas
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2012
Assuntos:
Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3439974/
https://ncbi.nlm.nih.gov/pubmed/22455878
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/gb-2012-13-3-r23
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