Bulged DNA substrates for identifying poxvirus resolvase inhibitors

Resolvase enzymes that cleave DNA four-way (Holliday) junctions are required for poxvirus replication, but clinically useful inhibitors have not been developed. Here, we report an assay for resolvase cleavage activity based on fluorescence polarization (FP) for high-throughput screening and mechanis...

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Bibliographische Detailangaben
Hauptverfasser: Culyba, Matthew, Hwang, Young, Attar, Sana, Madrid, Peter B., Bupp, James, Huryn, Donna, Sanchez, Luis, Grobler, Jay, Miller, Michael D., Bushman, Frederic D.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2012
Schlagworte:
Methods Online
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3439875/
https://ncbi.nlm.nih.gov/pubmed/22581770
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks325
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