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In Vitro Induction of Neospora caninum Bradyzoites in Vero Cells Reveals Differential Antigen Expression, Localization, and Host-Cell Recognition of Tachyzoites and Bradyzoites

We report on an optimized method for the in vitro culture of tissue cyst-forming Neospora caninum bradyzoites in Vero cells and the separation of viable parasites from host cells. Treatment of tachyzoite-infected Vero cell cultures with 17 μM sodium nitroprusside for 8 days severely scaled down para...

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Publicat a:Infect Immun
Autors principals: Vonlaufen, Nathalie, Guetg, Nicole, Naguleswaran, Arunasalam, Müller, Norbert, Björkman, Camilla, Schares, Gereon, von Blumroeder, Daniela, Ellis, John, Hemphill, Andrew
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 2004
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC343979/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14688139/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/IAI.72.1.576-583.2004
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