Insertion of a rabbit beta-globin gene sequence into an E. coli plasmid.
Double stranded DNA has been synthesized in vitro from rabbit globin messenger RNA and elongated with homopolymeric dG tails. An E. coli plasmid was cleaved by EcoRI. The cohesive ends were repaired and dC tails added, to permit reconstitution of the EcoRI sites upon annealing with the dG elongated...
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| Veröffentlicht in: | Nucleic Acids Res |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1975
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC343603/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/802513/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/2.12.2365 |
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