A new method of DNA denaturation mapping.
Described is a new method for DNA denaturation mapping utilizing glyoxal (ethanedial) to stabilize the denatured regions. The extent of glyoxal reaction can be easily and sensitively measured using an assay based on the intercalation of ethidium into duplex DNA. Thus denturation maps can be produced...
Guardat en:
| Publicat a: | Nucleic Acids Res |
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| Autor principal: | |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1975
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC343571/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1052528/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/2.11.2049 |
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