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The binding sites for tRNA on eukaryotic ribosomes.
We have studied the non-enzymic binding of phe-tRNA to ribosomes from rat liver using deacylated tRNA to inhibit binding to the P-site and puromycin (5 x 10-minus3M) to inhibit binding to the A-site. We conclude that at a low concentration of magnesium ions (10mM) phe-tRNA is bound only at the A-sit...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1975
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC343504/ https://ncbi.nlm.nih.gov/pubmed/1098024 |
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