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The fractionation of t-RNA on N,N′-bis(3-aminopropyl)-piperazine substituted-Sepharose

An anion exchange agarose has been prepared by modifying sepharose 6B with N,N′-bis (-3-aminopropyl) piperazine. This material (BAPP-Sepharose) has been used for the fractionation of t-RNA from E.coli by column chromatography. The results obtained with gram quantities of crude t-RNA at pH 4.6 and pH...

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Autors principals: Leberman, Reuben, Giovanelli, Ruth, Acosta, Zenobio
Format: Artigo
Idioma:Inglês
Publicat: 1974
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC343407/
https://ncbi.nlm.nih.gov/pubmed/10793731
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