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Use of phosphorus oxychloride in synthesizing nucleotides and oligonucleotides
Procedures are described for phosphorylating protected nucleotides, oligonucleotides and phosphoramidate oligonucleotide derivatives at the 3′-hydroxyl group. The conditions (phosphorylation with phosphorus oxychloride and pyridine in dioxane followed by hydrolysis with aqueous pyridine) are suffici...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1974
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC343360/ https://ncbi.nlm.nih.gov/pubmed/10793743 |
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