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Specific fragmentation of T7 phage DNA at low-melting sites.
A method has been developed for selective fragmentation of T7 DNA at AT-rich regions. The molecules have been subjected to complete digestion with single-strand-specific SI endonuclease after fixation of DNA AT-rich regions in the denatured state by glyoxal. The treatment resulted in three fragments...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1977
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC343221/ https://ncbi.nlm.nih.gov/pubmed/593901 |
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