Specific fragmentation of T7 phage DNA at low-melting sites.

A method has been developed for selective fragmentation of T7 DNA at AT-rich regions. The molecules have been subjected to complete digestion with single-strand-specific SI endonuclease after fixation of DNA AT-rich regions in the denatured state by glyoxal. The treatment resulted in three fragments...

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Bibliografiske detaljer
Main Authors: Pavlov, V M, Lyubchenko, Y L, Borovik, A S, Lazurkin, Y S
Format: Artigo
Sprog:Inglês
Udgivet: 1977
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC343221/
https://ncbi.nlm.nih.gov/pubmed/593901
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